Published literature shows that nasal secretions have been collected from the nasal mucosa by modifications of a filter paper/synthetic matrix method for many years (Alamet al. J ImmunolMethods. 1992; 155(1):25-9). Inflammatory markers have been measured in samples collected by these methods. The nose has often been investigated to understand mechanisms of rhinitis and airway inflammation. This study aims to address previously un-validated sampling techniques and investigate whether the nasal cycle might influence total protein yields.

A.Feneley1,2, M. Rainer1, F. Haider1, B. Narayanswamy1, J. Stenning1, C. Mordin1, S. Greenaway1, G.W. Clarke1,3

The nose as a research tool Intra subject variability in nasal sampling